FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16446055
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA43900.025.4172889
TTAGATC22700.019.9790748
GCCACTC74100.015.641148
AGCCACT89750.012.9939147
AAGCCAC99350.011.7383726
TAATTAG56800.010.3926485
GGCCTAA28850.08.48411875-79
CCGTTAA55500.08.3034764
CGTTAAA58350.08.1426125
TACAAAG157250.08.0627622
ACAAAGC158250.07.9628273
CTCACTA30450.07.754415515-19
ATACAAA177600.07.37008431
AATTAGA78200.06.9962866
AACACTA41450.06.9469617
TAGATCG62950.06.632769
ACACTAT46450.06.50915058
ATTAGAT95050.06.3618967
AAAGCCA211150.05.96633535
TTAATTA126700.05.68324474
Produced by FastQC (version 0.11.5)