FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13606316
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTG59450.026.519719
TTTAACT73550.019.9678827
TTAACTT80900.019.3995888
CTAGCTC17100.017.68046220-24
TTTTAAC96200.015.4167436
TCTAGCT20800.014.5353820-24
TTCTAGC25450.011.99274220-24
CCTTATT135450.011.4455881
GCTCTAA33900.09.25836525-29
ATTTTAA173600.09.1237525
CTTATTT177850.08.95074752
TTTCTAG36800.08.68582415-19
TATTTTA212350.07.63101674
TAGCTCT46400.07.322585620-24
AGCTCTA59350.06.42829220-24
CTTGCTA51750.06.426260510-14
CTCTAAA56300.06.086197425-29
TAAGATC53350.05.93811780-84
AGTGTAG58000.05.2373333110-114
CTAAAAC63700.05.220955425-29
Produced by FastQC (version 0.11.5)