FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15960981
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTG46050.027.0448769
TTTAACT67500.018.6640957
TTAACTT70050.018.292988
CTAGCTC14050.016.6007520-24
TCTAGCT17400.013.73560620-24
TTCTAGC20100.012.46356320-24
TTTTAAC108850.012.037686
CCTTATT111450.011.5216911
CTTATTT139050.09.738262
TTTCTAG28950.08.90284615-19
ATTTTAA156300.08.751745
GCTCTAA31200.07.75217925-29
TATTTTA184000.07.55376054
CTTGCTA42400.06.620736610-14
CTCTAAA46500.05.603956725-29
TTATTTT311350.05.15743063
TAGCTCT53650.05.12569420-24
ACCCCCC33752.3318007E-65.02922531
Produced by FastQC (version 0.11.5)