FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15637464
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT614160.3927491056094518TruSeq Adapter, Index 5 (97% over 38bp)
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC480440.3072365186580126No Hit
CCCTTTTCCACACGTGAGCCGATTTTTGGGACGTGGGGGATTTGCCCAGA223010.14261263846874403No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG187780.12008340994422115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA293300.035.616189
AGAGCAC308150.033.876458
GATCGGA320900.032.3938941
TCGGAAG334250.031.5007383
CCACACG118550.030.181928
TTAACCG74400.028.5557693
ATCGGAA382550.027.5215952
TCCACAC136850.026.408947
AAGAGCA402250.026.2021587
GTTTAAC86950.025.1824251
TAACCGG87400.024.2997954
GAAGAGC448050.023.7165726
GGAAGAG451900.023.6101025
TTTAACC92750.023.231052
CGGAAGA472200.022.4426354
CGGGGGT95750.021.7295688
CCCTTTT183850.020.1793521
GGGGGTG113150.019.342439
TTCCACA190600.019.3392266
CCGGGGG132350.016.5364277
Produced by FastQC (version 0.11.5)