FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15429643
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA136100.07.3006129
AACACTA36150.06.9710477
AGAGCAC145800.06.8149068
GATCGGA190250.05.90476941
ACACTAT43351.8189894E-125.8132268
TCGGAAG198050.05.5985523
CACTATA49151.0913936E-115.2737229
ATCGGAA240750.05.23361252
GTCACTA40800.05.223504525-29
AAGAGCA215950.05.16793167
Produced by FastQC (version 0.11.5)