FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15429643
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA36300.06.9417477
GATCGGA180500.06.1882631
CACTATA48900.05.8892689
ACACTAT43451.8189894E-125.79947148
TCGGAAG195700.05.70227583
AGAGCGT197150.05.6238558
AAGAGCG224800.05.3484457
ATCGGAA233450.05.11942962
Produced by FastQC (version 0.11.5)