FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16509657
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTTCAATCTCGTAT210020.12721039570961407TruSeq Adapter, Index 10 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA210500.017.2393449
GATCGGA233400.015.4576551
AGAGCAC236250.015.1774858
TCGGAAG292850.012.6125413
ATCGGAA310150.012.1411652
AAGAGCA337900.011.101767
GGAAGAG382650.09.7843525
CGGAAGA415400.09.1516184
AGTCACT86800.08.4607925-29
GAAGAGC490500.07.809356
CTCGTAT99600.07.330085840-44
CAGTCAC103150.07.189496525-29
TCACTGG105100.06.891690330-34
GTCACTG110700.06.71216425-29
CCAGTCA116950.06.41501925-29
TCTCGTA116200.06.369677540-44
CTAGATC16150.06.0631294140-144
TCGTATG132000.05.80360945-49
CACACGT139500.05.59490410-14
CACGTCT141650.05.489650710-14
Produced by FastQC (version 0.11.5)