FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16509657
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATCGCTGTGTAGATCT225040.13630810137363847Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA231400.015.3545591
AGAGCGT282400.012.8490858
TCGGAAG291750.012.4372993
ATCGGAA309600.011.9295142
TAGATCT65350.011.14919240-44
AAGAGCG352300.010.5449247
GAGCGTC391850.09.8297019
GGAAGAG384000.09.7119165
GTGTCTA80000.08.98146125-29
TGTGTAG82900.08.91048440-44
CGGAAGA419050.08.7793314
AGATCTC90700.08.38229745-49
GTAGATC93250.08.06046740-44
CTGTGTA93850.07.978306335-39
GAAGAGC490050.07.84525786
AGTGTCT101150.07.445127525-29
AGAGTGT106700.07.01738525-29
GAGTGTC114600.06.63415625-29
GCTGTGT117850.06.561246435-39
CTATCGC120200.06.39695330-34
Produced by FastQC (version 0.11.5)