FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15413516
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGCCTCGGTGTAGATCT384710.24959263026035072Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA218400.027.9560451
TCGGAAG249100.024.2215983
AGAGCGT272400.022.2026448
ATCGGAA288150.021.3638742
GAGCGTC285550.021.2810369
AAGAGCG308750.020.1017027
CGGAAGA366900.016.8961564
GGAAGAG369400.016.840285
GAAGAGC376750.016.3206396
TAGATCT96100.013.05140440-44
AGTGTCA116150.010.84804225-29
GAGTGTC114750.010.71686325-29
GTAGATC128900.09.98727740-44
AGAGTGT128500.09.88389125-29
GTAGGGA131350.09.55980215-19
GTGTCAG133200.09.50270325-29
TGTAGGG136250.09.19486215-19
TAGGGAA143000.09.03272715-19
TCATTAA141600.08.91864465-69
AGATCTC144800.08.82099545-49
Produced by FastQC (version 0.11.5)