FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16663098
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACTC63000.022.9708738
CCACTCA69000.021.5994789
AAGCCAC106000.014.2624356
AGCCACT117800.013.2628367
ACAAAGC188350.08.4856643
CCCTTAA99650.07.8023734
CACCACT63550.07.70398337
CTCACTA42950.07.243891715-19
TACAAAG229950.07.11019332
ATACAAA247050.06.85329771
ACCACTA66650.06.58948858
GTTCCCT125400.06.346931
ACACACT50750.06.32975250-54
ACAACAC84250.06.3235413
AAAGCCA262450.06.3090155
GGCCCCT49800.06.186996110-114
CCTAGAC15450.06.058342120-124
TACACAC54300.05.99551550-54
ACTCACT52900.05.99029715-19
TAGCCAC57350.05.95056625-29
Produced by FastQC (version 0.11.5)