FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16968757
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCAT81300.016.2934278
TATAACC97050.013.6484836
AACCATG105650.013.5602829
ATAACCA105300.012.5111247
CATATTA109550.012.3511971
TTATAAC125450.010.8450165
CTACGCG83950.07.71781164
TATCTAC91550.07.0719661
TCTACGC102700.07.00969843
ATCTACG105150.06.50631572
ATTATAA231750.06.3369864
TATTATA245100.06.3148773
TACGCGC114300.06.1089225
TAGGATG47550.05.90451435-39
ATATTAT271850.05.6160032
CCAATTA59550.05.391998315-19
CGATTTA67450.05.2296936
TCGGAAG236150.05.0604593
Produced by FastQC (version 0.11.5)