FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14706194
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGTC33600.018.4270298
TTAGTCC36250.017.4780489
CCCTACT42800.015.979978
CTCCCTA46700.014.6454546
CCTTAGT45450.014.4146347
TCCCTAC59150.011.806287
TCCTTAG63400.011.1284056
CCTACTT79100.09.1931259
GCTCCCT92150.08.0470745
GTATGCT93450.07.6948621
TACCTCC107300.07.24653632
CTCCTTA99300.06.9601425
CTACCTC108850.06.87311651
TATGCTC116550.06.67141442
TGCTCCC110900.06.4919684
Produced by FastQC (version 0.11.5)