FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12485442
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT49150.023.4373178
AACACTA48550.023.133797
CACTATA53200.021.247099
TACCGAG80200.014.8178283
AAACACT78250.014.72127156
CAAACAC86500.013.2339875
CGAGATA93850.012.7345476
ACCAAAC93450.012.4856363
ACCGAGA104500.011.7857314
CCAAACA106200.011.1223364
GAGATAA125450.09.7564027
CCGAGAT125350.09.5918385
TAGCCCT51950.09.43792
GCCCTTA49050.08.9571034
TACCAAA141850.08.488662
ATACCGA159900.08.2970062
ATACCAA160850.08.1212731
AACATGC96100.07.42786172
ACATGCT90200.07.42593863
CATGCTT92550.07.31523234
Produced by FastQC (version 0.11.5)