FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19371019
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTCA72000.013.0966496
GTCCCCT90300.011.327642
AGTCCCC82800.011.0910411
CCCCTCT106500.09.4685524
CTCTCAA104300.09.3169367
CCCTCTC122400.08.8800815
TCCCCTC145150.07.5433883
TAGGGGG34509.968062E-105.84201347
TCTCAAA181250.05.7584678
TTCACTC97800.05.6672038
ATTCACT105500.05.25365927
TCACTCC104150.05.25253539
CTCAAAA207500.05.13402849
GATTCAC100000.05.03871066
ACTCGAA112300.05.0120431
Produced by FastQC (version 0.11.5)