FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17199793
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTCACAATCTCGTAT215470.12527476348116517TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA161750.024.2080979
AGAGCAC168600.023.6514768
GATCGGA186850.020.9492681
TCGGAAG206200.019.3782223
ATCGGAA234500.017.2238252
AAGAGCA251500.016.3133417
GGAAGAG299200.013.7135815
GAAGAGC300450.013.7034486
CGGAAGA319750.012.6971474
AGTCACT72350.010.90384325-29
GTCACTC84500.09.26786625-29
CAGTCAC98400.08.45610325-29
TCACTCT96250.08.40615530-34
CACACGT101100.08.13050510-14
CCAGTCA106600.07.98119425-29
CTCGTAT101750.07.85299440-44
CACGTCT110100.07.583561410-14
CACTATA58250.07.16701139
CACTCTT114400.07.08507130-34
TCGTATG112850.07.067712345-49
Produced by FastQC (version 0.11.5)