FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15155866
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT313720.2069957599255628TruSeq Adapter, Index 5 (97% over 37bp)
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC160240.10572803955907237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA184700.032.93849
AGAGCAC203350.029.986928
GATCGGA215550.028.9040531
TCGGAAG271400.023.5833023
AAGAGCA289550.021.5569927
ATCGGAA298800.021.0106032
GAAGAGC335600.018.7706246
AACACTA55800.018.3208987
GGAAGAG361350.017.7717195
ACACTAT58500.016.8599918
CGGAAGA398750.016.2317714
CACTATA68600.014.7981579
AGTCACA111800.010.77966925-29
CAGTCAC119400.010.66030625-29
GTCACAC111550.010.53276325-29
TCACACA115900.010.10015430-34
AAACACT104500.09.9895126
CAAACAC108250.09.8429765
CTCGTAT118050.09.78257240-44
TCTCGTA121950.09.62322140-44
Produced by FastQC (version 0.11.5)