FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19014991
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC12450.016.19202875-79
GGACTAG13000.015.28545275-79
GACTAGC12950.015.23327775-79
CGCTAGT4854.7199544E-513.3592354
TCTAGCT15950.013.000003100-104
CTAGCTC13750.012.671892105-109
CTAGATC14250.012.52814625-29
TTCTAGC15950.012.368058100-104
CTAGCCT14100.012.2536880-84
GGCCTAG14400.012.0976720-24
GAGCTAG14850.011.53896125-129
CCTAGAT14800.011.38162125-29
AGCTAGA15200.011.273262125-129
GCTAGAA15500.011.05507125-129
GATCTAA17000.010.84029425-29
GCCTAGA19500.09.45048720-24
GAAGCTT100700.09.3651979
GGTCCCG101900.09.1173871
CTAATAG22100.08.60018940-44
TTTCTAG24200.08.38968100-104
Produced by FastQC (version 0.11.5)