FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19014991
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC14800.012.35607175-79
GGACTAG15900.011.50124875-79
GACTAGC16400.011.326200575-79
CTAGCCT16350.011.27207680-84
TCTAGCT17550.010.994822100-104
GGCCTAG16800.010.71629520-24
CTAGCTC16850.010.682384105-109
CTAGATC16750.010.6598825-29
GAGCTAG17350.010.290302125-129
CCTAGAT15700.010.27220825-29
AGCTAGA17950.09.946338125-129
TTCTAGC20250.09.8844100-104
GATCTAA20050.09.4081125-29
GCTAGAA18500.09.028013125-129
GAAGCTT101550.08.5782569
CTAATAG23100.08.41487440-44
GCCTAGA23050.08.18548220-24
CTAGTGG8150.0055998797.95056
GGTCCCG108850.07.949491
TTTCTAG26950.07.4270535100-104
Produced by FastQC (version 0.11.5)