FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15866112
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT61150.038.9699828
AACACTA73150.032.084997
CACTATA85750.027.7914839
AAACACT89300.026.4436286
CAAACAC97450.024.5275925
CCAAACA110100.022.2985524
ACCAAAC121650.020.1818083
GCCCTTA69600.010.2408394
TACACTC53850.09.305114115-119
TTTAGGC56400.09.242043560-64
TAGCCCT85550.09.0889142
CCCCTAA55350.09.02720190-94
TACCAAA280950.08.9946922
ATACCAA299350.08.8544881
ACGTATA61100.08.460598140-144
CCCTAAA62900.08.19546590-94
CTATAGC59700.08.17658210-14
TTAGGCA66650.07.799119560-64
GCTCTCA67950.07.58612115-19
TATTTAG104600.07.29612069
Produced by FastQC (version 0.11.5)