FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20978217
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTTG200400.010.1664651
GGTGTTA88550.09.5318911
GGTGAAA252850.09.1223341
GGTGTAG175450.09.0409231
GTCCCTA25550.09.0162333
GGTATAA195050.08.9904351
GGTGTTT149300.08.8212161
GGTAAAA260850.08.5636161
GGTGTTC206600.08.5582621
GGTGTAC128500.08.5503421
GGTATTT257500.08.5337391
GGTGTGA86600.08.2341321
GGTGTAA145200.08.1181681
GGTATAG170050.08.0871371
GGTGTGG142550.07.96282151
GGTGTCA86900.07.95451071
GGTGTCG214450.07.93961521
GGTGTAT135100.07.80948261
GGTATTG246500.07.58622741
GGTGTGT71650.07.5149341
Produced by FastQC (version 0.11.5)