FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15238167
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA18650.014.2883043
TAGGCTA26150.09.0924552
CGACTAA17152.928573E-108.8188713
CGTCTAA23455.456968E-127.9852463
TTAGGCT33850.07.7362141
CTATACT41000.07.6290291
GTACGTA34050.07.61770772
GACTCTA27450.07.6119262
AGATCTA42400.07.307042
CACGTAA53600.07.2558253
ACTCTAA28055.456968E-127.18923953
GTAGCTA28105.456968E-127.17944052
TACGTAA39250.06.9727123
AGGCTAA38900.06.8503053
TACTAAC22002.678771E-76.54734374
CTCCTAA35501.8189894E-126.4920143
GAGCTAA28603.3433025E-96.29552273
CGTACGT44450.06.2186551
ATACTAA53300.06.21567733
TCACGTA58250.06.18461942
Produced by FastQC (version 0.11.5)