FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14979145
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC878850.5867157304372179No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG474540.31680045823710234No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA312470.20860336154032824No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA247850.16546338258959373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA16950.025.0507073
TAGGCTA32500.015.2792982
AGATCTA33400.012.7128592
CGTACGT45000.012.1927211
GTACGTA44450.012.1430332
TTAGGCT45150.010.5532391
GTTCCTA66750.010.1347792
TAGAGTA51300.09.6798672
TACGTAA58900.09.4083423
AGGCTAA62500.08.4058283
ACAGTGT50500.08.1486021
CAGATCT62200.07.89256431
GAGCTAA50200.07.74154663
GTAGAGT65150.07.4243761
GTAGCTA49600.07.2548092
CAGTGTA53000.07.1967712
TTCCTAA98250.07.17845873
TGAGCTA60200.06.69467072
GATCAGT65500.06.61316821
AGCTAAC32857.2759576E-126.57239344
Produced by FastQC (version 0.11.5)