FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15559262
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAAGCCACTCAACTCACTATATAGCCACTATACCATATAGCTCTAC288360.18533012684020617No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC273530.17579882644819528No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGAGTAAATCTCGTAT189790.12197879308157417TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA77950.054.5734029
GCCACTC90600.047.350848
AAGCCAC117050.036.4049076
AGCCACT142100.030.1392567
ACAAAGC162900.026.7755783
TAGGCTA42600.025.6815552
GATCTAA39150.025.1869833
TACAAAG178950.024.4545232
TTAGGCT46200.023.128951
ACAGTGT47000.022.7352661
GTAGAGT59700.021.0431691
AGATCTA47900.020.586022
AAAGCCA211900.020.550595
GTTCCTA61450.020.3804932
CGTACGT41100.019.8505631
GAGCACA167850.019.5976739
GTACGTA44450.018.9452972
GAGCTAA49500.018.7573643
ATACAAA258250.018.1443161
CAGTGTA56000.017.7369272
Produced by FastQC (version 0.11.5)