FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16277120
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA17350.026.55373
TAGGCTA22600.016.8831962
TAGCTAA21450.013.759453
GTTCCTA34400.013.3939532
TTAGGCT25400.013.3563031
TAGAGTA28900.012.9536682
AGGCTAA31450.012.3599773
AGTGTAA39450.012.0431883
GTAGCTA23700.011.8468672
GTACGTA25400.011.6208362
TTCCTAA39750.011.5901053
TACGTAA24200.011.3034953
CTACTAA26350.010.9275783
GAGCTAA26500.010.0507953
AGAGTAA44700.09.8235143
CAGTGTA49350.09.4822952
AGATCTA55450.09.2181562
ACAGTGT49900.08.6790631
CACGTAA33400.08.6210083
TCAGTAA42450.07.97011663
Produced by FastQC (version 0.11.5)