FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16665104
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT47900.028.5659771
GTGAGTA50400.027.9989572
TGAGTAG57600.024.3759943
TAGGAGG62450.023.1747557
GAGTAGG67150.021.7670824
TAGCATG59900.018.7520738
GTCTCTT75750.015.306441
AGGAGGG106800.014.2253428
GGAGGGG111350.013.9027199
GTAGGAG120950.012.0848616
CTAGGAG32600.09.49712650-54
AGTAGGA160900.09.4870245
AGTCTCT41500.08.1560661
TTAGCAT176950.07.2430417
CTCTTTA169900.07.0350133
AGCATGA192750.06.57460649
AACCCTA55150.06.058089385-89
CTAACTA53600.05.99089125-129
TTCTAGG56000.05.811547350-54
CTTTAGC217250.05.76686335
Produced by FastQC (version 0.11.5)