FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005089868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005089868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16192640
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT83400.028.5717288
CCCCTTA56150.026.0262414
CACTATA92950.025.8685239
AACACTA103450.023.1037337
CAAACAC106250.022.4943945
AAACACT111250.021.6132976
GTACCCC79600.018.1857241
TACCCCT84100.017.9758112
CCAAACA139400.016.938554
ACCAAAC143000.016.9148623
ACCCCTT105250.014.979153
CCCTTAT109700.013.6496625
AGTGAGT49000.012.0521731
CCTTATT133200.011.4579316
TACCAAA227350.011.1458432
TATTTAG130350.011.1009359
GTGAGTA53600.011.0132192
GTCTCTT70950.09.9476671
ATACCAA280350.09.3507931
CTATAGC67950.07.45868110-14
Produced by FastQC (version 0.11.5)