FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005090997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005090997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15082787
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTAT8656385.739244345226117TruSeq Adapter, Index 9 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1264950.0109.225959
GATCGGA1288550.0108.6231461
TCGGAAG1293800.0108.4129943
AGAGCAC1280350.0107.985298
ATCGGAA1322250.0106.11762
AAGAGCA1332500.0104.422337
CGGAAGA1377600.0101.811184
GAAGAGC1368800.0101.737246
GGAAGAG1391800.0100.7594155
CTCGTAT1036700.026.20646340-44
TCTCGTA1055050.025.92946240-44
TCGTATG1035900.025.74874745-49
TATCTCG1056700.025.70500840-44
TCAAGTA1091300.025.6899535-39
AAGTATC1104100.025.5799635-39
AGTCACC1095850.025.49971825-29
CAAGTAT1103200.025.43372735-39
CAGTCAC1095400.025.395825-29
CGTATGC1113100.025.27184745-49
GTATCTC1056800.025.24338240-44
Produced by FastQC (version 0.11.5)