FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005090998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005090998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15082787
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGCCTCGGTGTAGATCT8708655.773899744125539Illumina Single End PCR Primer 1 (96% over 32bp)
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG190240.12613053542425548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1270750.0108.940521
TCGGAAG1271700.0108.528663
ATCGGAA1296100.0106.5605852
AGAGCGT1287050.0106.248038
AAGAGCG1308400.0104.5583347
GAGCGTC1314950.0103.89249
CGGAAGA1359750.0101.345434
GAAGAGC1354850.0101.133066
GGAAGAG1386650.099.473195
TAGATCT1063350.026.1484840-44
AGTGTCA1057350.026.00337425-29
CGTATCA1065300.025.94128260-64
GAGTGTC1067150.025.78211825-29
GTATCAT1078700.025.70312160-64
GTGTCAG1070750.025.63223325-29
GTAGATC1093350.025.56795340-44
GTGTAGA1107500.025.23478540-44
AGAGTGT1100750.025.12723725-29
ATCATTA1107200.025.12083460-64
CATTAAA1112150.025.11061165-69
Produced by FastQC (version 0.11.5)