FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18463357
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA68150.017.5392638
CACCACT87500.013.9074597
CCACTAC89050.012.4524819
CCTAGAC28550.011.448802120-124
GTTACGT63250.011.1586581
ACAACAC118900.011.0801743
ACGTTAA61800.010.9497114
ACACCAC128700.010.0707796
GCCACTA48000.09.1507716
TACGTTA74850.08.7523963
CTAGACC38450.08.538445120-124
ATACAAC154850.08.3169251
AACACCA172850.07.91504435
TACAACA174550.07.87754442
CTAACAC44250.07.6139425-29
ACCCTAG11700.07.50706510-14
CGTTAAA96400.07.24542435
CCACTAT63450.07.0360557
ATCCTAG50250.06.992007115-119
CACTATA69700.06.81836568
Produced by FastQC (version 0.11.5)