FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18463357
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTA69950.017.598568
CACCACT83400.015.0192967
ACAACAC112900.011.9249123
CCACTAC92400.011.2969829
CCTAGAC28650.010.603656120-124
ACACCAC132250.09.7981296
ACGTTAA62950.09.6070734
ATACAAC155550.09.2430571
GTTACGT59650.08.9316521
GCCACTA46200.08.5701246
CTAGACC39200.08.153887120-124
TACAACA175850.08.1064432
TACGTTA73850.07.9941243
CTAACAC44600.07.845093325-29
AACACCA179150.07.51434565
CGTTAAA94900.06.6754855
ATCCTAG47100.06.6638594115-119
CAACACC218200.06.40109874
ACCCTAG13100.06.264752410-14
AGCCACT72850.05.929095
Produced by FastQC (version 0.11.5)