FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14714494
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA18850.014.8906043
AGATCTA36550.08.4672072
AGTGTAA58600.07.49188853
TAGGCTA35300.07.33984472
TAGAGTA43300.07.14726072
TTAGGCT44900.06.7529761
AGGCTAA49050.06.6028673
GAGCTAA36650.06.48035763
ACAGTGT59500.06.43059441
GAGCACA114650.06.15219749
AGAGCAC121650.05.7982468
CAGTGTA72450.05.76167352
TGAGCTA42954.0017767E-115.5298052
TAGCTAA47552.3646862E-115.29756833
GTAGAGT60200.05.15660951
CTACTAA46403.5470293E-105.11864573
Produced by FastQC (version 0.11.5)