FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17671571
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTAG8150.011.484107135-139
CTAGTTA7700.011.03217185-89
GATCTAG8100.010.31051980-84
GCTAGTC9450.09.90624575-79
CTAGTCC10000.09.79346775-79
AGCTAGA10850.09.15652450-54
GAGCTAG9600.08.99893950-54
CTAGATC14250.08.789433140-144
GGCTAGT10750.08.57430775-79
AGGCTAG11000.08.510365575-79
ACTAGAT11850.08.019865135-139
ATCTAGT9900.07.998843785-89
GCTAGAA13150.07.773987350-54
CTATTCT73600.07.23726465
ACTATTC74700.07.2270524
TCTACTA68800.06.96192841
AACTAGA15300.06.6820297135-139
TACTATT85100.06.43228343
CTACTAT89250.06.3755612
TCTAGTT13950.06.192653785-89
Produced by FastQC (version 0.11.5)