FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17671571
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTA7550.011.82356285-89
TAACTAG7400.011.304441135-139
GATCTAG8350.010.51870380-84
CTAGTCC9850.08.916224575-79
AGGCTAG9900.08.43490475-79
GCTAGTC10700.08.20792675-79
GGCTAGT10600.08.01370875-79
ATCTAGT11100.07.912440385-89
ACTAGAT12100.07.86704135-139
GAGCTAG10150.07.51680950-54
CTAGATC15050.07.1857114140-144
AGCTAGA12300.07.13918150-54
TCTAGTT13850.06.653249385-89
ACTATTC68250.06.3280744
AACTAGA15100.06.304053135-139
CTATTCT72750.06.2332685
TCTACTA67550.06.05949541
GCTAGAA14650.05.993988550-54
TACTATT82650.05.83518933
CTACTAT88550.05.28532742
Produced by FastQC (version 0.11.5)