FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15535667
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGGCATCTCGTAT259160.16681613991855TruSeq Adapter, Index 9 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA14250.030.8096563
GAGCACA238050.020.7716129
AGAGCAC269750.018.570818
GATCGGA288950.017.364621
TCGGAAG315800.015.8851953
ATCGGAA324050.015.7028782
AAGAGCA326850.015.4806757
AGATCTA36950.012.8558662
TAGGCTA37100.012.609892
CGGAAGA416350.012.4464584
GGAAGAG416500.012.3212115
GAAGAGC430000.011.7336376
TTAGGCT45500.010.31347751
TAGAGTA56350.09.1962432
AGTGTAA61450.08.7843833
CTCGTAT105800.08.74931140-44
ACAGTGT61000.08.6396511
AGTCACG110950.08.55221825-29
CACGTAA48000.08.39689353
AGGCTAA56350.08.3021643
Produced by FastQC (version 0.11.5)