FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15414399
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC36200.029.6361397
TAGACCC42750.024.2532678
GTTAGAC46900.022.107196
TAGTTAG62150.017.4936774
AGACCCC61200.016.8238629
GCAACAC115400.016.7214097
CAACACT117600.016.2861428
AACACTA152900.012.7615359
AGTTAGA85100.012.6067165
GTTTCAG164150.012.012761
TAGACTC41150.09.413063135-139
CAGCAAC216900.09.2616845
ATGTAGT129250.09.165041
GACTCTA45600.08.494464135-139
AGCAACA243700.08.4204096
GTAGTTA139050.08.129693
TCAGCAA276150.07.79599334
ACACTAG52100.07.517827570-74
TGTAGTT150800.07.3526442
TTTCAGC304450.07.07097632
Produced by FastQC (version 0.11.5)