FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15414399
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC41450.027.4425227
TAGACCC43700.026.1942378
GTTAGAC48400.023.7994066
TAGTTAG64800.018.5548274
GCAACAC118100.017.6782637
AGACCCC66800.017.5671449
CAACACT124400.016.551448
AGTTAGA89200.013.8820975
AACACTA158800.013.1473319
GTTTCAG170500.012.589511
ATGTAGT131650.09.6843241
TAGACTC44350.09.5478325135-139
CAGCAAC224250.09.4706775
GTAGTTA144300.08.981143
AGCAACA249350.08.97930056
GACTCTA47900.08.659804135-139
TGTAGTT155600.08.4703642
ACACTAG53600.08.0056470-74
TCAGCAA290750.07.92402174
TTTCAGC305550.07.5662962
Produced by FastQC (version 0.11.5)