FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15740878
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG181100.013.5988243
GATCGGA184800.012.6779371
AGAGCGT200250.011.5771728
ATCGGAA233700.010.4151162
GAGCGTC243850.09.8024469
AAGAGCG241450.09.6613017
GGAAGAG289400.08.6572635
GAAGAGC300750.08.3546436
CGGAAGA313000.08.0062394
TAGATCT75000.06.758153440-44
TAGGGAA74950.06.570996315-19
AGTGTGA78350.06.32225325-29
AGAGTGT83300.06.13671325-29
GTAGGGA80350.06.07561915-19
GAGTGTG86300.06.006814525-29
TCATTAA82450.05.93781165-69
TGTAGGG93700.05.302201315-19
AAGAGTG100050.05.19568225-29
GTGTAGA107050.05.178699540-44
Produced by FastQC (version 0.11.5)