FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15971365
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC19700.012.7902767
TAGTTAG26600.010.5555314
GTCTCAG46650.010.5358171
GTTAGAC24400.010.326936
TAGACCC29650.08.4980928
AACACTA60650.08.4276329
AGTTAGA37200.07.9347625
CAACACT66350.07.59512958
TCTCAGC84800.06.3710762
ACACTAG17150.05.70963170-74
Produced by FastQC (version 0.11.5)