FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15971365
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC21700.017.3292857
TAGTTAG24550.015.5323214
GTTAGAC28800.013.5593316
TAGACCC31000.012.3637778
AGTTAGA38250.010.9645935
GTCTCAG46550.08.8401051
GTAGTTA58200.07.4164853
AGACCCC66400.06.97058739
AACACTA63950.06.78528649
CAACACT65600.06.50406938
TCTCAGC90650.05.31789062
ACACTAG17800.05.254758470-74
CTCAGCA111000.05.0552363
Produced by FastQC (version 0.11.5)