FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16413932
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAATT64650.010.3573292
GATCAAT68300.09.92003251
CTAGTCC6800.08.68244475-79
GCTAGTC7400.08.173058575-79
TCAATTC88350.07.741883
GGCTAGT8000.07.740081375-79
TAACTAG8150.07.598357135-139
GAGCTAG7350.07.444844750-54
CTAGATC9350.07.0857277140-144
ACTAGAT9450.06.2482862135-139
AGCTAGA10500.06.17138450-54
CAATTCA57400.05.89465574
GATCTAG5453.3657125E-75.812734680-84
Produced by FastQC (version 0.11.5)