FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14772824
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACTATTCTTTCCCCTGCACTGTACCCCCCAATCCCCCCTTTTCTTTT1364090.9233779540052734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGTG217350.097.251671
CTATTCT223250.091.826965
ACTATTC223850.091.8059164
CTACTAT230350.088.981472
CTTGTGG243250.087.135542
TACTATT250300.082.030393
AAAGGAC269550.078.562669
TGTGGAA274550.077.081034
TCTACTA269600.076.407231
TGGAAAG316350.066.941666
GTGGAAA316400.066.840095
GGAAAGG319850.066.411697
GAAAGGA341050.062.448948
TCTTTCC346400.059.3462379
TTGTGGA406100.052.2625433
TATTCTT444600.046.6763466
TTCTTTC462250.044.860548
ATTCTTT465050.044.6702427
GATCTAG147450.026.9686880-84
GCTAGTC168100.026.14029775-79
Produced by FastQC (version 0.11.5)