FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14714163
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAG11300.014.27251350-54
GCTAGTC12850.013.89279775-79
GGCTAGT13200.013.4153675-79
CTAGTCC14550.012.46748175-79
AGGCTAG13250.012.278172575-79
AGCTAGA15250.010.953402550-54
GCTAGAA17700.010.00674350-54
TCTTGTG106700.08.7805481
CTAGAAA23900.07.953108350-54
CTTGTGG126100.07.8782752
AAAGGAC139200.06.8775749
TTGTGGA160100.06.25004633
TTAGAGC30000.05.999296745-49
TGTGGAA175200.05.71115834
GTGGAAA208100.05.0848715
Produced by FastQC (version 0.11.5)