FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14994897
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA51600.019.6750099
GCCACTC71950.014.41043958
AGCCACT96800.011.3061247
AAGCCAC99050.010.4677546
CCGTTAA50400.010.2860234
CGTTAAA56000.09.2574215
TACAAAG144600.07.91677472
ACAAAGC140100.07.60622363
CTCACTA29800.07.29642615-19
ATACAAA169250.06.9136431
GTTAAAT77150.06.90623436
TCCGTTA83150.06.58107573
GTGAGTA35601.8189894E-126.47171642
TAACCCC48000.06.000189
AAAGCCA196800.05.9635945
CCCCTAA43300.05.3873744100-104
ACTCACT41550.05.371680315-19
AGTGAGT36351.8897481E-85.36512661
TACACAC45850.05.21336650-54
CTACACA44300.05.168242550-54
Produced by FastQC (version 0.11.5)