FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14963519
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAATC110350.014.549416
TACCAAT115600.014.1377715
ATCACAC72350.012.040928
TCACACC80750.011.7689719
CCAATCT137600.011.6157547
TTACCAA141250.011.5197884
GTTTTAC153350.010.6520791
GAATCAC89800.010.2623426
CAATCTC167500.010.0580538
AATCACA96250.09.2754257
TTTTACC194150.08.93880752
AATCTCC178200.08.9287699
TTTACCA200050.08.8537423
TGAATCA105750.08.5102545
CGAGCTA42300.07.897515140-144
CCCACTA46450.07.68784315-19
CCACTAA45650.07.50714320-24
ATGAATC128450.07.2867484
CACTAAT56300.06.24050820-24
CCCCACT63050.06.029167715-19
Produced by FastQC (version 0.11.5)