FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15717542
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGC25550.0101.96433
GGACTAG26300.099.333052
ACTAGCC27350.095.778114
CTAGCCT28100.094.499565
CGGACTA71900.036.39071
AGCCTTA95600.027.857677
TAGCCTT101950.026.1202146
CCTTATT119150.022.5933849
GCCTTAT119950.022.2625338
CTAGCTC25300.020.72137630-34
TCTAGCT30050.018.06532925-29
TTCTAGC33300.016.56164725-29
TTTCTAG37250.014.88275825-29
GCTCTAA48400.011.45653230-34
TAACTTG53050.010.69444315-19
CTTGCTA58800.09.57628620-24
CTCTAAA63500.09.14048230-34
TAGCTCT65100.08.8494630-34
TAGATCT71500.08.096508115-119
AGTGTAG67650.08.08928110-114
Produced by FastQC (version 0.11.5)