FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17601306
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA16600.031.6495273
TAGGCTA24300.016.5857142
TAGAGTA30750.013.80890752
GAGCTAA27950.013.3897963
AGATCTA44000.012.4312082
TTAGGCT34950.012.1882811
GTTCCTA40250.011.8013182
AGGCTAA38400.010.683073
GTACGTA32350.010.2337782
TAGCTAA22300.09.6820833
GTAGAGT44800.09.50849151
AGAGTAA52300.09.4951073
TTCCTAA46550.09.4311063
TACGTAA35850.09.2346653
CTACTAA31600.09.11014653
AGTGTAA44650.08.8653053
GTAGCTA24550.08.5015662
AGCTAAC21203.274181E-118.1475654
CACGTAA37500.08.06065753
TGAGCTA52850.07.7621552
Produced by FastQC (version 0.11.5)