FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17336066
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA272300.034.077789
AGAGCAC292900.032.2709248
TCGGAAG367100.027.378583
GATCGGA371550.027.0631081
AAGAGCA376500.025.717187
ATCGGAA411550.024.8258132
GGAAGAG445550.022.2665355
GAAGAGC436150.022.2017026
CGGAAGA507300.019.7127514
CACACGT204450.09.33800710-14
CACGTCT209000.09.12093810-14
ACACGTC232050.08.37625710-14
ACGTCTG233750.08.22927115-19
CGTCTGA245250.08.08409615-19
ACTCCAG201250.08.05620720-24
GTCTGAA256750.07.69396515-19
CTGAACT244450.07.639351415-19
AGCACAC254200.07.59540610-14
TCTGAAC256600.07.546962715-19
TGAACTC254150.07.308490820-24
Produced by FastQC (version 0.11.5)