FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17336066
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATTACTCGCGTGTAGATCTCGGT389030.22440500630304477Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG350150.022.1235833
GATCGGA353150.021.9031241
AGAGCGT332200.021.1951548
AAGAGCG365250.020.5387887
ATCGGAA392350.019.8471762
GAAGAGC419900.018.0199766
GGAAGAG426050.017.8443475
GAGCGTC411950.017.441489
CGGAAGA491600.015.7578774
TTACTCG125350.011.61295825-29
TAGATCT129300.011.36975340-44
ACTCGCG151250.09.88166230-34
ATTACTC157600.09.565474525-29
CTCGCGT156600.09.51648730-34
TACTCGC154850.09.41016830-34
TCGCGTG159750.09.31081230-34
CGCGTGT166300.09.24713230-34
GTAGATC171300.09.07811135-39
AGATCTC171600.08.94465740-44
GTGTAGA175700.08.79340535-39
Produced by FastQC (version 0.11.5)