FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11449393
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC137550.12013737322144502No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTTAGCATCTCGTAT122430.10693143295893502TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA154000.015.8008129
AGAGCAC155250.015.5808498
GATCGGA159000.015.1952851
TCGGAAG186500.013.2483693
AAGAGCA203850.012.1487397
ATCGGAA206850.011.8752872
GAGTTAG45850.010.552299530-34
CGAGTTA45850.010.42667730-34
AGTTAGC46100.010.33926935-39
GAAGAGC240850.010.1038246
GGAAGAG257700.09.8622495
GTTAGCA48300.09.77888735-39
CGGAAGA277100.08.9124
TTAGCAT63200.07.90633435-39
TAGCATC68550.07.64639435-39
CACGAGT66800.07.28597130-34
GGGGGGG44650.06.93735843
CTCGTAT71850.06.81409940-44
AGTCACG71850.06.813605325-29
TCTCGTA79650.06.454145440-44
Produced by FastQC (version 0.11.5)