FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11449393
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAGCCGGTGTAGATCT123400.1077786394440299Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA166200.014.5647591
AGAGCGT171550.013.7169478
TCGGAAG189900.012.6160953
GAGCGTC195800.012.12949
ATCGGAA207350.011.8682982
AAGAGCG201350.011.7226827
GAAGAGC243500.09.901626
GGAAGAG257000.09.4091735
TAGATCT55100.09.14028540-44
CGGAAGA280150.08.6294054
GAGTGTC72800.06.74049925-29
AGAGTGT76150.06.689634325-29
GTAGATC80850.06.602936740-44
AGTGTCA76650.06.40193525-29
GTGTAGA88950.05.969303640-44
GTAGGGA89200.05.61405915-19
TAGGGAA91550.05.611416315-19
AGATCTC89650.05.456972645-49
GTGTCAT96450.05.356255525-29
TCATTAA96600.05.31759265-69
Produced by FastQC (version 0.11.5)